DNA Loading Buffer

Published on Author Giorgio Gilestro

NEB Recipe: 1X Gel Loading Dye, Blue (6X):

  • 2.5 % Ficoll 400
  • 11 mM EDTA
  • 3.3 mM Tris-HCl
  • 0.017 % SDS
  • 0.015 % Bromophenol Blue
  • pH 8.0 @ 25°C

Simpler alternative

Makes 100 ml. Store at room temperature (indefinitely).

  • 0.25 g bromophenol blue (m.w. 669.96)
  • 0.25 g xylene cyanol (m.w. 538.60)
  • 50.00 g sucrose (m.w. 342.30) (or 50 ml of glycerol)
  • 1.00 ml 1M Tris (pH 8.0)

If using sucrose:
Dissolve bromophenol blue, xylene cyanol, sucrose and Tris in 60 ml deionized or distilled water. Add deionized or distilled water to make 100 ml total solution.

 

If using glycerol:
Dissolve xylene cyanol, bromophenol blue, and Tris in 49 ml of deionized or distilled water.
Stir in 50 ml of glycerol to make 100 ml total solution.