Common Buffers – TAE

Published on Author Giorgio Gilestro

10X TAE Electrophoresis

  • 48.4 g of Tris base [tris(hydroxymethyl)aminomethane]
  • 11.4 mL of glacial acetic acid (17.4 M)
  • 3.7 g of EDTA, disodium salt
  • deionized water
  1. Dissolve the Tris, glacial acetic acid and EDTA in 800 ml of deionized water.
  2. Dilute the buffer to 1 L. You do not need to sterilize the solution.

Store the bottle of 10X buffer solution at room temperature.