Fly Resources

Published on Author Anne Petzold

Some helpful websites and tools for some in silico research on fly genes

  • FlyBase, http://flybase.org/ – Ties to together resources about the individual genes, including GBrowse gene locus viewer, predicted function according to InterPro and UniProt, lists of associated papers, etc.
  • FlyAtlas 2,¬†http://flyatlas.gla.ac.uk/flyatlas/index.html – Gene expression profile, based on in situ hybridization (reliability?)
  • Virtual Fly Brain, http://www.virtualflybrain.org/site/vfb_site/home.htm – Ties together transgenes/phenotypes/gene expression profile etc. for anatomical locations in the fly brain
  • Interactive fly, http://www.sdbonline.org/sites/fly/aimain/1aahome.htm – by Society for Developmental Biology, mostly anatomical and developmental resources on flies

Some helpful tools for experimental planning

  • FlyPrimerBank, http://www.flyrnai.org/FlyPrimerBank – Primers for almost every fly gene, some already tested and commented on (paper on FlyPrimerBank by Hu et al. 2013)
  • FlyC31, http://www.frontiers-in-genetics.org/flyc31/ – Resources on PhiC31 integrase system implemented in flies
  • Crispr design tools: Zhang lab @ http://crispr.mit.edu/ (also: Google discussion group), Crispr Design tool @ http://tools.flycrispr.molbio.wisc.edu/targetFinder/, e-crisp @ http://www.e-crisp.org/E-CRISP/
  • Further Crispr resources: OxfordFlyCrispr @ http://groups.mrcfgu.ox.ac.uk/liu-group/useful-links/oxfcrispr/oxfcrispr, Cambridge Crispr/CrisprFlyDesign @ http://www.crisprflydesign.org/about-us/ (also: Google discussion group)

Stock Centers

  • VDRC RNAi stock center, http://stockcenter.vdrc.at/control/main – mainly RNAi lines and associated lines, e.g. Dicer2
  • Bloomington Fly Stock Center, http://flystocks.bio.indiana.edu/ – like the default stock center w/ big selection, esp. of Gal4 driver lines

Miscellaneous

  • Addgene, https://www.addgene.org/ – extensive non-profit depository for plasmids/vectors
  • LifeTechnologies, http://www.lifetechnologies.com – Helpful How-To’s and Tips, e.g. Buffer Recipes @ http://www.lifetechnologies.com/uk/en/home/life-science/cell-analysis/cellular-imaging/ihc/zymed-buffer-recipes.html
  • Cold spring Harbor Protocols, http://cshprotocols.cshlp.org/