NEB Recipe: 1X Gel Loading Dye, Blue (6X):
- 2.5 % Ficoll 400
- 11 mM EDTA
- 3.3 mM Tris-HCl
- 0.017 % SDS
- 0.015 % Bromophenol Blue
- pH 8.0 @ 25°C
Simpler alternative
Makes 100 ml. Store at room temperature (indefinitely).
- 0.25 g bromophenol blue (m.w. 669.96)
- 0.25 g xylene cyanol (m.w. 538.60)
- 50.00 g sucrose (m.w. 342.30) (or 50 ml of glycerol)
- 1.00 ml 1M Tris (pH 8.0)
If using sucrose:
Dissolve bromophenol blue, xylene cyanol, sucrose and Tris in 60 ml deionized or distilled water. Add deionized or distilled water to make 100 ml total solution.
If using glycerol:
Dissolve xylene cyanol, bromophenol blue, and Tris in 49 ml of deionized or distilled water.
Stir in 50 ml of glycerol to make 100 ml total solution.