Author: Giovanni Morelli

Sequencing in Tube format

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When submitting sequences to MWG, follow directions as in the attached PDF document (here) Briefly, for plasmidic DNA from minipreps: Make sure your mini is of good quality, that is obtained with a Qiagen kit and of at least 200ng/ul (standard yield for kit preparation) Use 1.5ml eppendorf tubes with the proper sticky label on … Continue reading Sequencing in Tube format

Plasmid miniprep

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Procedure Shake the culture tube to resuspend the cells. Label 2mL tubes and pour about 2ml of the cell suspension into each tube. Close the caps and place the tubes in a centrifuge (remember to balance the centrifuge by putting the tubes opposite one another) and spin at 11.000 rpm for 2 minutes. Withdraw and … Continue reading Plasmid miniprep