Some helpful websites and tools for some in silico research on fly genes
- FlyBase, http://flybase.org/ - Ties to together resources about the individual genes, including GBrowse gene locus viewer, predicted function according to InterPro and UniProt, lists of associated papers, etc.
- FlyAtlas 2, http://flyatlas.gla.ac.uk/flyatlas/index.html - Gene expression profile, based on in situ hybridization (reliability?)
- Virtual Fly Brain, http://www.virtualflybrain.org/site/vfb_site/home.htm - Ties together transgenes/phenotypes/gene expression profile etc. for anatomical locations in the fly brain
- Interactive fly, http://www.sdbonline.org/sites/fly/aimain/1aahome.htm - by Society for Developmental Biology, mostly anatomical and developmental resources on flies
Some helpful tools for experimental planning
- FlyPrimerBank, http://www.flyrnai.org/FlyPrimerBank - Primers for almost every fly gene, some already tested and commented on (paper on FlyPrimerBank by Hu et al. 2013)
- FlyC31, http://www.frontiers-in-genetics.org/flyc31/ - Resources on PhiC31 integrase system implemented in flies
- Crispr design tools: Zhang lab @ http://crispr.mit.edu/ (also: Google discussion group), Crispr Design tool @ http://tools.flycrispr.molbio.wisc.edu/targetFinder/, e-crisp @ http://www.e-crisp.org/E-CRISP/
- Further Crispr resources: OxfordFlyCrispr @ http://groups.mrcfgu.ox.ac.uk/liu-group/useful-links/oxfcrispr/oxfcrispr, Cambridge Crispr/CrisprFlyDesign @ http://www.crisprflydesign.org/about-us/ (also: Google discussion group)
Stock Centers
- VDRC RNAi stock center, http://stockcenter.vdrc.at/control/main - mainly RNAi lines and associated lines, e.g. Dicer2
- Bloomington Fly Stock Center, http://flystocks.bio.indiana.edu/ - like the default stock center w/ big selection, esp. of Gal4 driver lines
Miscellaneous
- Addgene, https://www.addgene.org/ - extensive non-profit depository for plasmids/vectors
- LifeTechnologies, http://www.lifetechnologies.com - Helpful How-To's and Tips, e.g. Buffer Recipes @ http://www.lifetechnologies.com/uk/en/home/life-science/cell-analysis/cellular-imaging/ihc/zymed-buffer-recipes.html
- Cold spring Harbor Protocols, http://cshprotocols.cshlp.org/