Gel Preparation:
Below shows the volume of agarose solution required to make the desired agarose gel for each unit tray size.
1. For a standard 0.7% agarose gel, add 0.7 grammes of agarose to 100 ml of 1x TAE or TBE solution. The same 1 x solution should be used in the tank buffer solution.
| Multi Sub Mini | Multi Sub Midi | Multi Sub Choice | Multi Sub Choice Stretch | ||||
| Tray | Vol for 5 mm gel | Tray | Vol for 5 mm gel | Tray | Vol for 5 mm gel | Tray | Vol for 5 mm gel |
| 7x7 cm | 25 ml | 10x7 cm | 35 ml | 15x7 cm | 52.5 ml | 15x20 cm | 150 ml |
| 7x10 | 35 ml | 10x10 cm | 50 ml | 15x10 cm | 75 ml | 15x25 cm | 187.5 ml |
| 15x15 cm | 112.5 ml |
2. Add the agarose powder to a glass bottle.
3. Add the appropriate amount of 1x TAE or TBE solution from the table above. To prevent evaporation during the dissolving steps below, leave cap loosely screwed on.
4. Dissolve the agarose powder by heating the agarose in a microwave oven. Overflows easily and very, very fast! The solution should be heated until all crystals are dissolved. This is best viewed against a light background. Crystals appear as translucent crystals. These will interfere with sample migration if not completely dissolved. The gel must be cooled to between 50°C and 60°C degrees before pouring.
Source: http://www.cleaverscientific.com/wp-content/uploads/2013/02/MSMINI-MIDI-Choice-Manual.pdf