Instructions according to Stockinger lab manual: http://www.oardc.ohio-state.edu/stockingerlab/Protocols/GlycerolStocks.pdf
Make a glycerol stock of plasmid
- pick a single colony from the transformation plate and grow an o/n culture in appropriate medium, e.g. LB amp
- put 0.5 ml of 80 % sterile glycerol in sterile screw cap microcentrifuge tube
- add 0.5 ml of o/n culture
- vortex
- freeze @ -80 DEG
- check with a digestion of a miniprep whether stock construct is correct
Take sample from glycerol stock
- take a sterile blue plastic scraper and scrape off a portion from the top of the stock
- plate the portion on an agar plate and grow o/n
- DON'T LET STOCK THAW!
80 % glycerol stock:
- 80 % glycerol
- 20 % ddH2O