| ID | Oligo Sequence | Len | TM | Tags | Template | Comments | Owner |
|---|---|---|---|---|---|---|---|
| pGG-1 | ACCAGATctGCGCCTTGTTGCTGTA | 25 | 59 | CG10638 HR genomic AKR | CG10638 – 3’UTR | HR | admin |
| pGG-2 | TCTACTACCTAgGTCAGATTCAAGAGC | 27 | 58 | CG10638 HR genomic AKR | CG10638 – 3’UTR | HR | admin |
| pGG-3 | GAACGGTCACgCTaGCCGAGAG | 22 | 60 | CG10638 HR genomic AKR | CG10638 – 5’UTR(B) | HR | admin |
| pGG-4 | GCTTTTGCATTTTGGtAcCTTGCAGC | 26 | 57 | CG10638 HR genomic AKR | CG10638 – 5’UTR(B) | HR | admin |
| pGG-5 | GCAGTTTGTTTTTgcATGCTTAGACCC | 27 | 58 | CG10638 HR genomic AKR | CG10638 – 5’UTR(A) | HR | admin |
| pGG-6 | CTCTCGGCtAGcGTGACCGTTC | 22 | 60 | CG10638 HR genomic AKR | CG10638 – 5’UTR(A) | HR | admin |
| pGG-37 | gtgccgtgccaaagatcgatgc | 22 | 58 | CG10638 genomic AKR | CG10638 | sequencing | admin |
| pGG-38 | CAGTACTATCCGTACTAGCAAGAG | 24 | 55 | CG10638 genomic AKR | CG10638 | sequencing | admin |
| pGG-39 | ggagagccgcgaattgacaaatc | 23 | 57 | CG10638 genomic AKR | CG10638 | sequencing | admin |
| pGG-40 | GCACTGCCGTAATTAAATTTGAAC | 24 | 52 | CG10638 genomic AKR | CG10638 | sequencing | admin |
| pGG-41 | GCTCGCTCCGACTGTTAAGCT | 21 | 56 | CG10638 genomic AKR | CG10638 | sequencing | admin |
| pGG-42 | GCGTTTCAGTTTCTGGTCGCCAA | 23 | 57 | CG10638 genomic AKR | CG10638 | sequencing | admin |
| pGG-43 | TGTCCCGAATCGCCTTGCCC | 20 | 57 | CG10638 genomic AKR | CG10638 | sequencing | admin |
| pGG-44 | GGTACCGTGCCAATATCGATCTG | 23 | 57 | CG10638 genomic AKR | CG10638 around P | sequencing | admin |
| pGG-45 | GGGCGTGGACTACATCTCTGATGA | 24 | 59 | CG10638 genomic AKR | CG10638 around P | sequencing | admin |
| pGG-46 | GCCATGGAAAAGCTGGTAAA | 20 | 49 | CG10638 rtPCR qPCR AKR | GC10638-A | admin | |
| pGG-47 | GGAATGACACCCAGACCAAC | 20 | 53 | CG10638 rtPCR qPCR AKR | GC10638-A | admin | |
| pGG-78 | CATTTGTACTTTTTGAGCCAGA | 22 | 49 | CG10638 rtPCR qPCR AKR | CG10638 – 3’UTR | admin | |
| pGG-79 | AGTGCTACACTGGGAGAAATTCA | 23 | 53 | CG10638 rtPCR qPCR AKR | CG10638 – 3’UTR | admin | |
| pGG-80 | TACTGTGTTACGGTGGTGATTGA | 23 | 53 | CG10638 rtPCR qPCR AKR | CG10638 – 5’UTR -A | admin | |
| pGG-81 | GGAATGACCAAAAGGAGTACAAA | 23 | 51 | CG10638 rtPCR qPCR AKR | CG10638 – 5’UTR -A | admin | |
| pGG-82 | CAATCTAAGTGCCTACGATGTGC | 23 | 55 | CG10638 rtPCR qPCR AKR | CG10638 – 5’UTR -B | admin | |
| pGG-83 | GATCAGTTGGTCGTGGAATGT | 21 | 52 | CG10638 rtPCR qPCR AKR | CG10638 – 5’UTR -B | admin | |
| pGG-90 | CTACCTAAGTCAGATTCAAGAGCAAGGCTTTTTCG | 35 | 63 | CG10638 rtPCR qPCR AKR | CG10638 3’UTR | corrects primer 2 | admin |
| pGG-91 | AGTGCTTTTGCATTTTGGCAGCTTGCAGCA | 30 | 61 | CG10638 rtPCR qPCR AKR | CG10638 5’UTR | corrects primer 4 | admin |
| pGG-92 | TTATCTCTCGGCCAGTGTGACCGTTCGA | 28 | 62 | CG10638 rtPCR qPCR AKR | CG10638 5’UTR | corrects primer 6 | admin |
| pGG-149 | gcgcgaatTCTTGATTTAAACGTGCAAAATGAAGC | 35 | 62 | CG10638 cDNA AKR | RH46018 | Cloning | admin |
| pGG-150 | gcgctctagaCGTGATACAGCAACAAGGCGC | 31 | 67 | CG10638 cDNA AKR | RH46018 | Cloning | admin |
| pGG-151 | TAGTTAAAGAAtTCTCGCTGTGAGGCTGCCG | 31 | 63 | CG10638 cDNA AKR | IP08405 | Cloning | admin |
| pGG-152 | gcgctctAGATTCTATCTTATACATTTACATAGTTACAC | 39 | 61 | CG10638 cDNA AKR | IP08405 | Cloning | admin |
| pGG-246 | CAGAAACTGAAACGCGAACA | 20 | 49 | CG10638 qPCR AKR | CG10638-B | RTPCR | Anne Petzold |
| pGG-247 | CGCCCAGTAGCTTTAATTGC | 20 | 51 | CG10638 qPCR AKR | CG10638-B | RTPCR | Anne Petzold |
| pGG-336 | TATATAGGAAAGATATCCGGGTGAACTTC- GGACAAGATCGCAGAAGGTG- GTTTTAGAGCTAGAAATAGCAAG | 77 | 72 | CG10638 gibson CRISPR genomic AKR | CG10638-RA, pCFD4 | Gibson Assembly | Alice French |
| pGG-337 | TATATAGGAAAGATATCCGGGTGAACTTC-ggcgatgctaataaatcagc – GTTTTAGAGCTAGAAATAGCAAG | 77 | 71 | CG10638 gibson CRISPR genomic AKR | CG10638-RB, pCFD4 | Gibson Assembly | Alice French |
| pGG-338 | ATTTTAACTTGCTATTTCTAGCTCTAAAAC- ATTCGCCACTGGGGCGTCCC- GACGTTAAATTGAAAATAGGTC | 77 | 71 | CG10638 gibson CRISPR genomic AKR | CG10638-RB, pCFD5 | Gibson Assembly | Alice French |
| pGG-339 | TAATACGACTCACTATA-GGCTACGAGATGCCAATTCT-GTTTTAGAGCTAGAAATAGC | 59 | 68 | CG10638 gibson CRISPR genomic AKR | CG10638, pCFD4 | in vitro transcription, whole gene | Alice French |
| pGG-340 | TAATACGACTCACTATA-GGATCTTCTATTCTGATGTA-GTTTTAGAGCTAGAAATAGC | 59 | 66 | CG10638 gibson CRISPR genomic AKR | CG10638, pCFD4 | in vitro transcription, whole gene | Alice French |
| pGG-343 | TAATACGACTCACTATA-GGACAAGATCGCAGAAGGTG-GTTTTAGAGCTAGAAATAGC | 59 | 69 | CG10638 gibson CRISPR genomic AKR | CG10638-RA | in vitro transcription | Alice French |
| pGG-344 | TAATACGACTCACTATA-ggcgatgctaataaatcagc-GTTTTAGAGCTAGAAATAGC | 59 | 68 | CG10638 gibson CRISPR genomic AKR | CG10638-RB | in vitro transcription | Alice French |
| pGG-345 | TAATACGACTCACTATA-GGGACGCCCCAGTGGCGAAT-GTTTTAGAGCTAGAAATAGC | 60 | 71 | CG10638 gibson CRISPR genomic AKR | CG10638-RB | in vitro transcription | Alice French |
| pGG-415 | GTCGGCTACGAGATGCCAATTCT | 23 | 57 | sgRNA CRISPR CG10638 | Allnighter whole sgRNA 1 | to be cloned into pCFD3 | Alice French |
| pGG-416 | AAACAGAATTGGCATCTCGTAGC | 23 | 53 | sgRNA CRISPR CG10638 | Allnighter whole sgRNA 1 | to be cloned into pCFD3 | Alice French |
| pGG-417 | GTCGGATCTTCTATTCTGATGTA | 23 | 51 | sgRNA CRISPR CG10638 | Allnighter whole sgRNA 2 | to be cloned into pCFD3 | Alice French |
| pGG-418 | AAACTACATCAGAATAGAAGATC | 23 | 48 | sgRNA CRISPR CG10638 | Allnighter whole sgRNA 2 | to be cloned into pCFD3 | Alice French |
| pGG-419 | GTCGGACAAGATCGCAGAAGGTG | 23 | 58 | sgRNA CRISPR CG10638 | Allnighter A sgRNA 1 | to be cloned into pCFD3 | Alice French |
| pGG-420 | AAACCACCTTCTGCGATCTTGTC | 23 | 55 | sgRNA CRISPR CG10638 | Allnighter A sgRNA 1 | to be cloned into pCFD3 | Alice French |
| pGG-421 | GTCGGCGATGCTAATAAATCAGC | 23 | 55 | sgRNA CRISPR CG10638 | Allnighter B sgRNA 1 | to be cloned into pCFD3 | Alice French |
| pGG-422 | AAACGCTGATTTATTAGCATCGC | 23 | 51 | sgRNA CRISPR CG10638 | Allnighter B sgRNA 1 | to be cloned into pCFD3 | Alice French |
| pGG-423 | GTCGGGACGCCCCAGTGGCGAAT | 23 | 64 | sgRNA CRISPR CG10638 | Allnighter B sgRNA 2 | to be cloned into pCFD3 | Alice French |
| pGG-424 | AAACATTCGCCACTGGGGCGTCC | 23 | 60 | sgRNA CRISPR CG10638 | Allnighter B sgRNA 2 | to be cloned into pCFD3 | Alice French |
| pGG-429 | CTTTATTTTCAGGGCGCCATGAAGCTCGCTCCGACT | 36 | 67 | gibson CG10638 | CG10638 RA | Gibson Cloning of CG10638 RA into pETM-11 for protein production. 5′ in frame with TEV cleavage site and His TAG | admin |
| pGG-430 | TCGAGTGCGGCCGCATTAAAACTCAATTGAAAAGGGGTAGTAC | 43 | 68 | gibson CG10638 | CG10638 RA | Gibson Cloning of CG10638 RA into pETM-11 for protein production. Amplifies endogenous stop | admin |
| pGG-431 | TCGAGTGCGGCCGCACTAGTACGGATAGTACTGATGATTC | 40 | 69 | gibson CG10638 | CG10638 RB | Gibson Cloning of CG10638 RB into pETM-11 for protein production. Amplifies endogenous stop. Use together with pGG-429 | admin |
| pGG-495 | CGTTAGAACGCGGCTACAAT | 20 | 51 | CG10638 | IP08705 – pOt2 vector | primer to sequence the IP08705- pOt2 vector | Giorgio Gilestro |
Oligo Database
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