| ID | Oligo Sequence | Len | TM | Tags | Template | Comments | Owner |
|---|---|---|---|---|---|---|---|
| pGG-332 | TATATAGGAAAGATATCCGGGTGAACTTC-GGCTACGAGATGCCAATTCT – GTTTTAGAGCTAGAAATAGCAAG | 77 | 71 | gibson CRISPR genomic AKR | CG10638, pCFD4 | Gibson Assembly | Alice French |
| pGG-333 | ATTTTAACTTGCTATTTCTAGCTCTAAAAC-TACATCAGAATAGAAGATCC – GACGTTAAATTGAAAATAGGTC | 77 | 67 | gibson CRISPR genomic AKR | CG10638, pCFD4 | Gibson Assembly | Alice French |
| pGG-334 | TATATAGGAAAGATATCCGGGTGAACTTC-GGTGCAATGATTTAGAATGC – GTTTTAGAGCTAGAAATAGCAAG | 77 | 70 | gibson CRISPR genomic | Yellow, PCFD4 | Gibson Assembly | Alice French |
| pGG-335 | ATTTTAACTTGCTATTTCTAGCTCTAAAAC-TGTGTAGGATTATGTTAACC – GACGTTAAATTGAAAATAGGTC | 77 | 67 | gibson CRISPR genomic | Yellow, PCFD4 | Gibson Assembly | Alice French |
| pGG-341 | TAATACGACTCACTATA-GGTGCAATGATTTAGAATGC-GTTTTAGAGCTAGAAATAGC | 59 | 67 | gibson CRISPR genomic | Yellow, CG3757 | in vitro transcription | Alice French |
| pGG-342 | TAATACGACTCACTATA-GGTTAACATAATCCTACACA-GTTTTAGAGCTAGAAATAGC | 59 | 66 | gibson CRISPR genomic | Yellow, CG3757 | in vitro transcription | Alice French |
| pGG-425 | TGCAAGAGTACAGTATATATAGGAAAGATATCCGGGTGAACTTCGGCTACGAGATGCCAATTCTGTTTTAGAGCTAGAAATAGCAAG | 88 | 73 | gibson CRISPR genomic AKR | Re-ordering 332 with extra 12bp on front | to be cloned into PCFD4 using gibson | Alice French |
| pGG-429 | CTTTATTTTCAGGGCGCCATGAAGCTCGCTCCGACT | 36 | 67 | gibson CG10638 | CG10638 RA | Gibson Cloning of CG10638 RA into pETM-11 for protein production. 5′ in frame with TEV cleavage site and His TAG | admin |
| pGG-430 | TCGAGTGCGGCCGCATTAAAACTCAATTGAAAAGGGGTAGTAC | 43 | 68 | gibson CG10638 | CG10638 RA | Gibson Cloning of CG10638 RA into pETM-11 for protein production. Amplifies endogenous stop | admin |
| pGG-431 | TCGAGTGCGGCCGCACTAGTACGGATAGTACTGATGATTC | 40 | 69 | gibson CG10638 | CG10638 RB | Gibson Cloning of CG10638 RB into pETM-11 for protein production. Amplifies endogenous stop. Use together with pGG-429 | admin |
Oligo Database
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