TOPO TA Cloning
Topo TA cloning is done following loose suggestions of the manufacturer. We currently adopt the kit K204040 from Invitrogen, which is not based on topoisomerase ligation but regular T4 ligation. Comes with “One Shot® aTOP10 Chemically Competent E. coli”. Briefly. Quantify PCR product. The kit suggests to use 10ng of DNA. We usually skip this … Continue reading TOPO TA Cloning