Single Fly Genomic DNA Prep
From Georg Dietzl in Barry Dickson’s Lab, IMP Vienna 12/2002 SQUISHING BUFFER (SB) 10 mM Tris-HCl pH8 1 mM EDTA 25 mM NaCl 200 g/ml Proteinase K Procedure 200 g/ml Proteinase K are added to the squishing buffer (SB) freshly from 20X stock every day. Place 1-2 (I always take 2) flies in a 0.5 … Continue reading Single Fly Genomic DNA Prep