Useful tools for TALEN assembly

Published on Author Wee Hon Ang

https://boglab.plp.iastate.edu/protocols

Includes:

1) Soft copy of the Golden Gate TALEN assembly protocol. http://www.addgene.org/static/cms/files/GoldenGateTALAssembly2011.pdf

2) an online tool with useful information about the plasmid sequences used in the Golden Gate TALEN kit and generation of sequences based on the RVD input. http://baolab.bme.gatech.edu/Research/BioinformaticTools/assembleTALSequences.html

3) an excel sheet that generates the reagents needed after you enter your RVDs. (attached and here) www.public.iastate.edu/~ajbog/shared/Golden_Gate_TAL_Assembly_form.xlsx